WebDec 24, 2024 · Abstract Hydrophobic interaction chromatography (HIC) is a chromatographic technique that mainly targets the separation of biomolecules (intact proteins, monoclonal antibodies, etc.) ... (BioPro HIC HT) non-porous polymethacrylate particles functionalized with a butyl chemistry were obtained from (YMC Europe, … Weberage DAR of 4. Typically, this 2 nd generation ADC is analysed by hydrophobic interaction chromatography (HIC). In this application note YMC’s latest hydrophobic …
BioPro HIC HT - YMC
WebYMC BioPro HIC HT. Analytical. Columns by Name. Select the name of the desired product line. Product names that begin with “YMC-Pack” are listed on the left and all others are listed on the right. YMC-Pack C4 (Butyl) WebOn the other hand, BioPro HIC HT eluted all peaks completely with the good separation, and another peak was partially separated on the peak #3. Additionally, it is noteworthy that the same DAR values were observed at any content of 2-propanol. 10% 2-propanol in Eluent B 15% 2-propanol in Eluent B BioPro HIC HT Competitor A HIC column … significance of fill factor in solar cell
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WebOct 26, 2024 · Use of 2 different YMC HIC columns. YMC’s BioPro HIC BF and BioPro HIC HT were used in order to determine the effect on intact protein retention. The proteins used for evaluation were cytochrome c, myoglobin, ribonuclease A, lysozyme, carbonic anhydrase, trypsinogen and α-chymotrypsinogen A in varying combinations. WebIn this application note YMC’s latest hydrophobic interaction chromatography column, BioPro HIC HT, was used. A mobile phase of sodium phosphate buffer at neutral pH with decreasing gradients of the lyotropic salt ammonium sulphate was used. The rigid 2.3 µm non-porous polymer particles are pressure tolerant up WebBioPro HIC HT. 4 YMC Biochromatography Columns Phase selection guide Proteins / Peptides SEC YMC-Pack Diol-60 YMC-Pack Diol-120 YMC-Pack Diol-200 YMC-Pack Diol-300 YMC-SEC MAB IEX BioPro IEX QA BioPro IEX QF BioPro IEX SP BioPro IEX SF RP YMC-Triart Bio C4 YMCbasic Meteoric Core C18 BIO YMC-Triart C18 YMC-Triart Bio … significance of finding a bird feather