Cisplatin hplc method

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August undefined, 2024

WebCisplatin, cis-diamminedichlorido-platinum(II), is an important therapeutic tool in the struggle against different tumors, yet it is plagued with the emergence 掌桥科研一站式科研服务平台 WebJul 13, 2016 · The HPLC method was successfully applied to in vitro studies and enabled us to quantify CDDP not only in the NaCl medium but also in the cellular lysates using only 0.02 mL of a sample. Our results clearly demonstrate that the intracellular CDDP concentrations in EHD1-overexpressing cells were lower than those in control cells.

Development and Validation of a Simple and Selective Analytical HPLC …

WebThe HPLC system (LC-20AD model, Shimadzu, Kyoto, Japan) consisted of two pumps (LC 20-AT model), an automatic injector (9SIL-20AD model), an oven (CTO-20AS model), a spectrophotometric detector (SPD-M20A model), and a computer equipped with the analysis software Shimadzu LC. WebSep 1, 2016 · An isocratic reversed-phase high-performance liquid chromatographic method with ultraviolet detection at 254 nm has been developed for the determination of doxorubicin in human plasma. Plasma... sharon gordon kansas city university

In vivo detection of DNA adducts induced by cisplatin using ... - PubMed

WebAreas covered: In this review, we have discussed about cisplatin and its liposomal formulations, focusing on different preparation methods and analysis approaches such as atomic absorption, mass spectroscopy, UV, electrochemical methods, and emphasizing on HPLC as one of the accurate and specific methods for determination of cisplatin … WebThe platinum content (4.91%) of Polycisplatin was determined by modified HPLC–UV method detailed in the literature. 19 Briefly, a known amount of Polycisplatin was dissolved in 0.5 M HCl solution and stirred so as to cleave the AA-Pt bond and release the cisplatin. The cisplatin was then treated with 100 µL of (10%) DDTC in 0.1 N NaOH and ... WebAug 15, 2007 · The HPLC method was developed for individual as well as simultaneous determination of two drugs namely carboplatin and paclitaxel in the presence of polymer … sharon gordon realtor

A Novel Analytical Method of Cisplatin Using the HPLC with a

Cisplatin determination by HPLC method. Chromatogram shows …

WebWe successfully determined cisplatin, hydroxo complexes, and OH-dimer by HPLC using a πNAP column. Thus the measurement of cisplatin (cis-diamminedichloro-platinum(II), cis-[PtCl2(NH3)2]) (CDDP) should be done using a πNAP column rather than a C18 column or aminopropylsilyl silica gel column. WebDec 1, 2024 · Tezcan et al. [70] described a HPLC-PDA method for monitoring the plasma concentration of free cisplatin in different types of cancer. Deproteinization with cold MeOH was used for sample extraction of cisplatin and NiCI 2 .6.H 2 O as IS. sharon gosling authorWebApr 24, 2024 · Doxorubicin hydrochloride. Several methods such as LC-MS, reverse phase HPLC and UV-Visible spectrometry have been reported and validated for quantitative analysis of doxorubicin hydrochloride in bulk and pharmaceutical dosage forms [12,13,14, 22, 23].All these methods require use of solvents, however due to unstable … sharon gornstein attorney

"WebMethods: A microemulsion method was employed to generate lipid coated cisplatin/oleanolic acid calcium carbonate nanoparticles (CDDP/OA-LCC NPs), and the loading concentration of CDDP and OA was measured by atomic absorption spectroscopy and HPLC respectively.Transmission electron microscopy (TEM) was used to examine … " - Cisplatin hplc method

Cisplatin hplc method

Determination of Liposomal Cisplatin by High-Performance

WebA high-performance liquid chromatographic (HPLC) method is described for the determination of residual levels of cisplatin from extracts of surfaces with very low … WebMaterials and Methods Chemicals. Reduced glutathione (GSH), glutathione oxidized, and g-glutamyl glutamate, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, solvents of analytical grade (methanol and glacial acetic acid) used for HPLC, thioctic acid (a-lipoic acid, oxidized form) and cisplatin were purchased ...

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WebNov 1, 2008 · Purpose: The purpose of this study was to develop and validate HPLC-UV method for the determination of the cisplatin, based … WebCisplatin hydrochloride is an important chemotherapeutic drug for cancer treatment. It has a low molar absorptivity in the UV region and has no fluorescence. Therefore, a selective …

WebCisplatin was the first of the platinum-based chemotherapeutic agents and therefore has been extensively studied as an antitumor agent since the late 1960s. Because this agent … WebCisplatin is an antineoplastic drug used for the treatment of cancer patients with a variety of different malignancies. Application Cisplatin may be used as an analytical reference standard for the determination of the analyte in plasma, cancer cell, and tumor samples by high-performance liquid chromatographic assay.

WebHigh-performance liquid chromatography of cisplatin The retention behavior of cisplatin on a variety of stationary phases has been investigated using aqueous mobile phases … WebTotal and free cisplatin concentrations were measured using a validated HPLC-UV method. Results: The highest concentration of total and free cisplatin was achieved at the end of the infusion in both regimens. Total cisplatin concentration declined 30 minutes after the end of infusion in both the groups. After 1hour of discontinuing cisplatin ...

WebMar 1, 2024 · Cisplatin is a platinum containing compound used for the treatment of different types of cancers. Quantitative determination of cisplatin has been carried out …

http://www.ijsit.com/admin/ijsit_files/ijsit_1.1.5.pdf sharon gossman neligh neWebHypoxia can induce chemoresistance, which is a significant clinical obstacle in cancer therapy. Here, we assessed development of hypoxia-induced chemoresistance (HICR) against free versus polymeric cisplatin micelles in a triple negative breast cancer cell line, MDA-MB-231. We then explored two strategies for the modulation of HICR against … population study times square new yorkWebderivitized cisplatin [21]. The objective of study was to develop and validate a direct and effective HPLC method for the simultaneous determination of etoposide and cisplatin which could be used in quality control analysis. The present work therefore is a direct method with good linearity and sensitivity for both drugs. O O O H HO H HO O O O O ... sharon gosnellWebJun 13, 2005 · Cisplatin, cisplatinum or cis-diamminedichloroplatinum (II) (CDDP) is a platinum-based chemotherapy drug used to treat various types of cancers, including sarcomas, some carcinomas (e.g. small cell lung cancer, and ovarian cancer), lymphomas and germ cell tumors. It was the first member of its class, which now also includes … population subdivisionWebHPLC Analysis of Cisplatin and Monohydrated Cisplatin on SeQuant ZIC-HILIC. Home Small Molecule HPLC HPLC Analysis of Cisplatin and Monohydrated Cisplatin on … sharon gothardWebJun 12, 2000 · In water, the antineoplastic drug cisplatin, cis-[PtCl2(NH3)2] (1) hydrolyses slowly to the aqua complexes cis-[Pt(NH 3) 2 Cl(H 2 O)] + (2) and, to a small extent, cis-[Pt(NH 3) 2 (H 2 O) 2] 2+ (3), which are thought to play an important role in the metabolism of cisplatin.HPLC is a useful technique for monitoring 2 and 3, but only if the … population subdivision 翻译Webinjected onto the HPLC system. Retention time of cisplatin, mono-chloride, OH-dimer, and none-chloride was 3.2, 3.4, 3.6, and, 4.3–6.6 min, respectively. Measurable ranges with this method were 1˜10˚5 to 4˜10˚3 M for cisplatin. Correlation coefﬁcient of the calibration curves of cisplatin was 0.999 (p<0.01). The within- and population suriname 2020