Flanking sequence tag
WebT-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics. The GABI-Kat population of T-DNA mutagenized Arabidopsis thaliana lines … Webflanking sequence A DNA sequence located adjacent to a gene, either upstream from its 5'-end or downstream from its 3'-end. Tags: Molecular Biology Related Biology Tools GenSmart™ Codon Optimization GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression.
Flanking sequence tag
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WebThe T-DNA flanking sequences (FST) have been isolated using a PCR amplification procedure and sequenced. Seven hundred plant DNA sequences have been obtained … WebFrom a single transgenic line harboring five Tnt1 transposon insertions, we generated a near-saturated insertion population in Medicago truncatula. Using thermal asymmetric interlaced-polymerase chain reaction followed by sequencing, we recovered 388 888 flanking sequence tags (FSTs) from 21 741 insertion lines in this population.
WebApr 9, 2009 · The plant genomic sequence flanking the insert is commonly designated as a FST. Large populations of tagged insertional mutants can be produced so that one or more insertions are available for... WebFlanking Sequence Tag: All FSTs: Only T-DNA: Only Tos17: Only Ac/Ds: Nothing: Region: All: Promoter: Gene: 3' UTR : Orientation: All: Reverse: Forward : Output: FASTA file: 1000 bp of sequence preceding the 5' end of each transcription unit. Gene sequences from TAIR10 release, including UTRs and introns. CDS sequences from TAIR10 release. …
WebThe 5′ flanking region of the human GR was first isolated and characterized by Zong et al. (57). A genomic clone of about 3.0 kb was isolated and sequenced. Computer analysis … WebJeong DH, An S, Park S et al (2006) Generation of a flanking sequence-tag database for activation-tagging lines in japonica rice. Plant J 45:123–132 Lee YS, Jeong DH, Lee DY et al (2010) OsCOL4 is a constitutive flowering repressor upstream of Ehd1 and downstream of OsphyB. Plant J 63:18–30
WebAfter sequencing, the data were placed in a flanking sequence tag (FST) database describing which mutant allele was present in which line. Analysis of the distribution of T-DNA insertions revealed a clear bias towards intergenic regions. Insertion sites appeared more frequent in regions in front of the ATG and after STOP codons of predicted genes.
WebMar 4, 2015 · The sequence analysis shows that these two T-DNAs insert the AT2G11740 gene in a head-to-head orientation. Therefore, we obtained two flanking sequences by using nested left-border specific primers (Figure 3(A)). On the contrary, no flanking sequences were amplified by using right-border primers. how can you tell if i had a strokeWebJun 8, 2024 · In addition, the conventional methods for the isolation of flanking sequence tags (FSTs) characterizing the T-DNA inserts, such as TAIL-PCR, adapter ligation-mediated PCR, and inverse PCR, are time-consuming, laborious, and expensive. In this study, Agrobacterium-mediated transformation system was optimized to generate T-DNA … how can you tell if it is a surge protectorThe 5′ flanking region is a region of DNA that is adjacent to the 5′ end of the gene. The 5′ flanking region contains the promoter, and may contain enhancers or other protein binding sites. It is the region of DNA that is not transcribed into RNA. Not to be confused with the 5′ untranslated region, this region is not transcribed into RNA or translated into a functional protein. These regions primarily function in th… how can you tell if guppies are pregnantWebThe flanking maneuver is a basic military tactic, with several variations. Flanking an enemy means attacking from one or more sides, at an angle to the enemy’s direction of … how many people were there in 1900WebMay 1, 2013 · A total of 246 566 flanking sequence tags from rice mutant libraries with T-DNA, Ds / dSpm, or Tos17 insertion have been collected and analyzed. The results show that, among 211 470 unique hits, inserts located in the genic region account for 68.16%, and 60.49% of nuclear genes contain at least one insertion. how can you tell if its a load bearing wallWebApr 9, 2013 · New insertions have been identified for a number of the segregating SALK, SAIL and WiscDsLox lines. These insertions have been identified by TDNA-Seq, a next-generation sequencing method for capturing T-DNA flanking sequence tags developed in the Ecker laboratory. Some of these have been isolated and donated as new lines … how many people were sent to the gulagWebOffer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days. Largest commercial ORF clones database, 70,000 next-day … how many people were saved on the titanic