Tthssb
Web(A and B) Representative single-molecule time traces (A) and average cross-correlation curves (B), for (dT)65+hp+3 alone and TthSSB-bound (dT)65+hp+3, obtained at 500 mM … WebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95°C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical purposes.
Tthssb
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WebA fixed quantity (20 pmol) of 5h-end fluoresceinlabelled oligonucleotide (dT) 30 was incubated with 20 pmol of TaqSSB or TthSSB (#) in 20 µl reaction mixtures in standard … WebssDNA Binding Proteins. Product Listing Product Overview. NEB offers highly thermostable ssDNA binding proteins that are ideal for nucleic acid amplification and sequencing (Tth …
WebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not … WebThermus thermophilus (TthSSB). The three proteins were found as dimers in solution. They bound in vitro to single-stranded DNA specifically over a temperature range of 4–80oC, and the wild-type protein could withstand incubation at 94oC for 2 min. Addition of TthSSB to PCR halved the elonga-tion time required for the DNA polymerases of
WebA fixed quantity (20 pmol) of 5h-end fluorescein-labelled oligonucleotides (dT) 30 , (dT) 60 , (dT) 70 or (dT) 76 was incubated with 0, 5, 20, 50 and 200 pmol of TthSSB in 20 µl … WebMay 12, 2009 · Use of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500x. The use of mutant TthSSB not only promoted the amplification of circular target DNA over the background but also could be used to enhance the amplification of circular targets over …
WebExcellgen Tth-SSB [EG-171] - Return to List . Next
WebMay 17, 2006 · We have overexpressed the native and mutant forms of TthSSB protein in Escherichia coli and purified them to homogeneity. In vitro, these proteins were found to … ios chooseWebfrom T.thermophilus strain HB8 (TthSSB) increases the effi-*To whom correspondence should be addressed. Tel: +81 45 508 7224; Fax: +81 45 508 7364; Email: [email protected] The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors The Author 2006. Published by Oxford University Press. ios choosing uiWebJun 1, 2005 · Here, we demonstrate the application of SSB-like protein from Thermus aquaticus (TaqSSB) in multiplex PCR of human Y-STR markers identification.The use of thermostable TaqSSB prevents or reduces primer dimmer formation, one of the particular problems known to cause inhibition of primer hybridization to the template and reduction … on the throne again songhttp://www.diva-portal.org/smash/record.jsf?pid=diva2:658627 on the tick meaninghttp://www.biotools.eu/documentospdf/DNA-Polymerase-030.pdf on the thundermansWebUse of the mutant TthSSB resulted in an enhancement of plasmid or mitochondrial DNA content in the amplified product by approximately 500×. The use of mutant TthSSB not only promoted the amplification of circular target DNA over the background but also could be used to enhance the amplification of circular targets over linear targets. Crown", on the throne kari jobe lyricson the ticket 意味